SEDIMENTATION, DIFFUSION, AND MOLECULAR WEIGHT OF CRYSTALLINE PITUITARY GROWTH HORMONE

Sedimentation, diffusion, and molecular weight of crystalline pituitary growth hormone.

The recent work of Fishman, Wilhelmi, and Russell (1,2) has made available a crystalline growth hormone isolated from the bovine anterior pituitary gland. It was of considerable interest to determine the sedimentation behavior of this protein in the ultracentrifuge and, at the same time, to obtain the molecular weight by this method. The amorphous preparation of Li, Evans, and Simpson (3) has n...

The sedimentation constant, diffusion constant and molecular weight of lactoglobulin.

Sedimentation and diffusion studies on insulin: the maximum molecular weight.

A new preparation of crystalline anterior pituitary growth hormone.

The isolation and identification of the anterior pituitary growth hormone as a pure protein were first achieved by Li, Evans, and Simpson (1, 2) in 194445. Their method is a beautiful example of the classical procedures of protein fractionation by the controlled use of salt and of hydrogen ion concentrations. It owes its distinction to the proof that the growth-promoting activity of anterior pi...

Sedimentation behavior of hypophyseal growth hormone.

In a previous publication, it was reported that solution of the hypophyseal growth hormone in acetate buffers results in an increment of its intrinsic viscosity with an accompanying increase of molecular weight (1). Later, Smith et al. (2) confirmed these observations in ultracentrifugal studies. The purpose of this present investigation has been to study the sedimentation behavior of the hormo...